Immunochemical detection of peanut (Arachis hypogaeaL.) proteins using chicken antibodies

The Proteins of the Peanut, Arachis Hypogaea.

The ravages of the boll weevil have made it unprofitable to grow cotton in various sections throughout the South. In many regions where the growing of cotton has been decreased, peanuts are now being grown as a supplementary crop. This has caused a large increase in the production of peanuts in the United States. A number of mills which in the past produced cottonseed oil are now using their pr...

Expression screening for interacting proteins using immunochemical detection.

Screening of cDNA expression libraries is a well established and frequently used method for cDNA cloning. Generally, cDNAs are inserted in expression vectors like Xgtl 1 or XZAP as fusions with the lacZ gene such that they are under the control of the lac promotor. After plating recombinant bacteriophages on an appropriate bacterial strain, expression of fusion proteins is induced (1). Librarie...

Advances in Arachis genomics for peanut improvement.

Peanut genomics is very challenging due to its inherent problem of genetic architecture. Blockage of gene flow from diploid wild relatives to the tetraploid; cultivated peanut, recent polyploidization combined with self pollination, and the narrow genetic base of the primary genepool have resulted in low genetic diversity that has remained a major bottleneck for genetic improvement of peanut. H...

The hydrophobic character of peanut (Arachis hypogaea) isoagglutinins.

Peanut seed lectin (PNA) is widely used to identify tumor-specific antigens on the eukaryotic cell surface. In this work PNA was purified by affinity chromatography, using a column containing glutaraldehyde-treated human erythrocytes, whereas PNA isoforms were purified by hydrophobic interaction chromatography using Phenyl-Sepharose. The affinity-purified PNA and its isoforms consist of four eq...

Analysis of genomic sequences from peanut (Arachis hypogaea)